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What S. pyogenes protein(s) are you expressing in E. coli for your recombinant vaccine candidate?
What function does your protein provide in S. pyogenes? Why do you think it will make a good GAS vaccine candidate?
Are there any concerns about negative side effects or potential toxicity based on the GAS protein you have chosen? Would you expect your protein to cause an infection or disease symptoms in the host or have some other negative impact? Justify your answer.
If your protein of interest proves to be only mildly immunogenic when tested, what could you do to improve its immunogenicity? (1-2 sentences is fine)
Do you expect to see a cellular or humoral immune response to your vaccine? What part of the immune response could you measure to determine whether or not the vaccine is effective?
What advantage(s) does your vaccine have compared to a live GAS vaccine? Provide at least one advantage.
What disadvantage(s) does your vaccine have compared to a live GAS vaccine? Provide at least one disadvantage.
What media component(s) are necessary to ensure that your gene of interest is expressed in the recombinant E. coli cells?
What media component(s) would you need to add to shut down the expression of your gene in the recombinant E. coli cells?(I've attached my previous diagram of recombinant DNA)